Campus Units
Veterinary Microbiology and Preventive Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
11-1980
Journal or Book Title
Journal of Bacteriology
Volume
144
Issue
2
First Page
752
Last Page
575
Abstract
Listeria monocytogenes cells suspended in brain heart infusion broth or in carbonated saline solution emitted light (chemiluminescence) that could be detected by a liquid scintillation spectrometer. This chemiluminescence was inhibited by superoxide dismutase and catalase but not by the hydroxyl radical scavengers mannitol and benzoate; it was also dependent upon and proportional to the carbonate ion concentration in the medium. Organisms suspended in carbonated saline solution which had ceased to chemiluminesce immediately began to chemiluminesce again when acetaldehyde was added but not when glucose, sucrose, or xanthine was added. Acetaldehyde-induced chemiluminescence was inhibited by suproxide dismutase and catalase but not by allopurinol. Our data indicate that the superoxide anion, hydrogen peroxide, and the carbonate ion are involved in chemiluminescence by L. monocytogenes. Chemiluminescence is apparently initiated by the extracellular generation of superoxide anon by this organism. The mechanism for the production of the superoxide anion is not known, but xanthine oxidase does not appear to be involved.
Copyright Owner
American Society for Microbiology
Copyright Date
1980
Language
en
File Format
application/pdf
Recommended Citation
Roth, James A. and Kaeberle, Merlin L., "Chemiluminescence by Listeria monocytogenes" (1980). Veterinary Microbiology and Preventive Medicine Publications. 184.
https://lib.dr.iastate.edu/vmpm_pubs/184
Included in
Veterinary Microbiology and Immunobiology Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comments
This article is published as Roth, J.A., and M.L. Kaeberle. 1980. Chemiluminescence by Listeria monocytogenes. J Bacteriol, 144:752-757. Posted with permission.