Campus Units
Veterinary Microbiology and Preventive Medicine, Veterinary Diagnostic and Production Animal Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
4-10-2019
Journal or Book Title
Frontiers in Microbiology
Volume
10
First Page
698
DOI
10.3389/fmicb.2019.00698
Abstract
Campylobacter jejuni is a leading cause of foodborne illnesses worldwide. As a microaerobic pathogen, C. jejuni is subjected to DNA damages caused by various stresses such as reactive oxygen species (ROS) and UV radiations. The base excision repair (BER) system plays an important role in preventing mutations associated with oxidative DNA damage, but the system remains poorly characterized in Campylobacter. In this study, a BER homolog encoded by cj0595c (named nth) in C. jejuni was analyzed for endonuclease III activity and for its role in maintaining genomic stability. It was found that inactivation of nth resulted in elevated frequencies of spontaneous fluoroquinolone-resistant (FQR) and oxidative stress resistant (OXR) mutants, compared with the wild-type strain in C. jejuni. Sequencing analysis of the FQR and OXR mutants revealed that the elevated mutation rates were associated with C → T or G → A transition in gyrA (FQR mutants) or perR (for OXR mutants). In an in vitro assay, a purified recombinant C. jejuni Nth protein demonstrated endonuclease III activity that recognized and excised the thymine glycol (Tg) base from a double stranded DNA. These findings indicate that Nth functions as a BER repair enzyme in C. jejuni and is important for the repair of DNA damage, protecting the bacteria from stresses encountered within a host and in the environment.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Dai, Lei; Xia, Jing; Sahin, Orhan; and Zhang, Qijing, "Identification of a nth-Like Gene Encoding an Endonuclease III in Campylobacter jejuni" (2019). Veterinary Microbiology and Preventive Medicine Publications. 209.
https://lib.dr.iastate.edu/vmpm_pubs/209
Included in
Veterinary Microbiology and Immunobiology Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comments
This article is published as Dai, Lei, Orhan Sahin, and Qijing Zhang. "Identification of a nth‐like Gene encoding an Endonuclease III in Campylobacter jejuni." Frontiers in Microbiology 10 (2019): 698. DOI: 10.3389/fmicb.2019.00698. Posted with permission.