Campus Units

Veterinary Diagnostic and Production Animal Medicine, Veterinary Microbiology and Preventive Medicine

Document Type

Article

Publication Version

Submitted Manuscript

Publication Date

1-3-2020

Journal or Book Title

bioRxiv

DOI

10.1101/2020.01.02.893479

Abstract

Small non-coding RNAs are involved in many important physiological functions in pathogenic microorganisms. Previous studies have identified the presence of non-coding RNAs in the major zoonotic pathogen Campylobacter jejuni, however, few have been functionally characterized to date. CjNC110 is a conserved ncRNA in C. jejuni, located downstream of the luxS gene which is responsible for the production of the quorum-sensing molecule autoinducer-2 (AI-2). In this study, we utilized strand specific high-throughput RNAseq to identify potential targets or interactive partners of CjNC110 in a sheep abortion clone of C. jejuni. This data was utilized to focus further phenotypic evaluation of the role of CjNC110 in growth, motility, autoagglutination, quorum sensing, and chicken colonization in C. jejuni. Inactivation of the CjNC110 ncRNA led to a statistically significant decrease in autoagglutination ability as well as increased motility when compared to wild-type. Extracellular AI-2 detection was decreased in ΔCjNC110, however, intracellular AI-2 accumulation was significantly increased with a concurrent increase in LuxS expression, suggesting a key role of CjNC110 in modulating the transport of AI-2. Notably, ΔCjNC110 also showed a significant defect in the ability to colonize chickens. Complementation of CjNC110 restored all phenotypic changes back to wild-type levels. The collective results of the phenotypic and transcriptomic changes observed in our data provide valuable insights into the pathobiology of C. jejuni sheep abortion clone and strongly suggest that CjNC110 plays an important role in regulation of energy taxis, flagellar glycosylation, cellular communication via quorum sensing, and chicken colonization in this important zoonotic pathogen.

Comments

This is a pre-print of the article Kreuder, Amanda J., Brandon Ruddell, Kathy Mou, Alan Hassall, Qijing Zhang, and Paul J. Plummer. "Small non-coding RNA CjNC110 influences motility, autoagglutination, AI-2 localization, and chicken colonization in Campylobacter jejuni." bioRxiv (2020). DOI: 10.1101/2020.01.02.893479. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

Published Version

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