Document Type

Article

Publication Date

11-2005

Journal or Book Title

Antimicrobial Agents and Chemotherapy

Volume

49

Issue

11

First Page

4681

Last Page

4688

DOI

10.1128/​AAC.49.11.4681-4688.2005

Abstract

In this study, a 101-kb IncF plasmid from an avian pathogenic Escherichia coli(APEC) strain (APEC O2) was sequenced and analyzed, providing the first completed APEC plasmid sequence. This plasmid, pAPEC-O2-R, has functional transfer and antimicrobial resistance-encoding regions. The resistance-encoding region encodes resistance to eight groups of antimicrobial agents, including silver and other heavy metals, quaternary ammonium compounds, tetracycline, sulfonamides, aminoglycosides, trimethoprim, and beta-lactam antimicrobial agents. This region of the plasmid is unique among previously described IncF plasmids in that it possesses a class 1 integron that harbors three gene cassettes and a heavy metal resistance operon. This region spans 33 kb and is flanked by the RepFII plasmid replicon and an assortment of plasmid maintenance genes. pAPEC-O2-R also contains a 32-kb transfer region that is nearly identical to that found in the E. coli F plasmid, rendering it transferable by conjugation to plasmid-less strains of bacteria, including an APEC strain, a fecal E. coli strain from an apparently healthy bird, a Salmonella enterica serovar Typhimurium strain, and a uropathogenic E. coli strain from humans. Differences in the G+C contents of individual open reading frames suggest that various regions of pAPEC-O2-R had dissimilar origins. The presence of pAPEC-O2-R-like plasmids that encode resistance to multiple antimicrobial agents and that are readily transmissible from APEC to other bacteria suggests the possibility that such plasmids may serve as a reservoir of resistance genes for other bacteria of animal and human health significance.

Comments

This article is from Antimicrobial Agents and Chemotherapy 49, no. 11 (November 2005): 4681–4688, doi:10.1128/AAC.49.11.4681-4688.2005.

Copyright Owner

American Society for Microbiology

Language

en

File Format

application/pdf