Document Type

Article

Publication Date

12-2012

Journal or Book Title

Infection and Immunity

Volume

80

Issue

12

First Page

4115

Last Page

4122

DOI

10.1128/​IAI.00752-12

Abstract

Extraintestinal Escherichia coli (ExPEC), a heterogeneous group of pathogens, encompasses avian, neonatal meningitis, and uropathogenic E. coli strains. While several virulence factors are associated with ExPEC, there is no core set of virulence factors that can be used to definitively differentiate these pathotypes. Here we describe a multiplex of four virulence factor-encoding genes, yfcV, vat,fyuA, and chuA, highly associated with uropathogenic E. coli strains that can distinguish three groups of E. coli: diarrheagenic and animal-associated E. colistrains, human commensal and avian pathogenic E. coli strains, and uropathogenic and neonatal meningitis E. coli strains. Furthermore, human intestinal isolates that encode all four predictor genes express them during exponential growth in human urine and colonize the bladder in the mouse model of ascending urinary tract infection in higher numbers than human commensal strains that do not encode the four predictor genes (P = 0.02), suggesting that the presence of the predictors correlates with uropathogenic potential.

Comments

This article is from Infection and Immunity 80, no. 12 (December 2012): 4115–4122, doi:10.1128/IAI.00752-12.

Copyright Owner

American Society for Microbiology

Language

en

File Format

application/pdf