Document Type

Article

Publication Version

Published Version

Publication Date

1-1989

Journal or Book Title

Veterinary Immunology and Immunopathology

Volume

20

Issue

2

First Page

119

Last Page

133

DOI

10.1016/0165-2427(89)90093-7

Abstract

The effects of in vitro and in vivo treatment of bovine polymorphonuclear leukocytes with recombinant bovine interferon-γ on in vitro bovine polymorphonuclear leukocyte functions and the survival of Brucella abortus were determined. Activation of neutrophils in vitro with interferon-γ resulted in enhanced production of O2 and myelopeoroxidase-H2O2-halide activity by neutrophils in the presence of B. abortus. The improved iodination responses were correlated with an enhanced ability to perform iodination in the presence of 5′-guanosine monophosphate and adenine which have previously been shown to contribute to inhibition of neutrophil myeloperoxidase-H2O2-halide activity by B. abortus. The ability of opsonized B. abortus to survive in the presence of neutrophils activated in vitro or in vivo was partially decreased by ∼10% of control when compared to survival rates within control phagocytes. These results suggest that activation of neutrophils with recombinant interferon-γ partially enhances their oxidative metabolic responses, resulting in a slightly enhanced ability to kill virulent B. abortus.

Comments

This article is from Veterinary Immunology and Immunopathology 20 (1989): 119, doi:10.1016/0165-2427(89)90093-7.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf