Document Type

Article

Publication Version

Published Version

Publication Date

9-1986

Journal or Book Title

Journal of Infectious Diseases

Volume

154

Issue

3

First Page

464

Last Page

470

Abstract

Brucella abortus releases two components (fractions 3b and 10) that inhibit the myeloperoxidase- H202-halide antibacterial system of bovine neutrophils by inhibiting degranulation. Fractions 3b and 10 were analyzed by high-performance liquid chromatography and thin-layer chromotography and compared with nucleotide and base standards. These investigations indicated that fraction 3b coeluted and co migrated with 5' -guanosine monophosphate (GMP), whereas fraction 10 coeluted and comigrated with adenine. We determined the biologic effects of GMP, adenine, and B. abortus fractions 3b and 10 on neutrophil functions. Iodination activity of neutrophils was inhibited to rv6507o of control by GMP (0.04 mg/ml) and by fraction 3b (0.04 mg/ml). Iodination was also suppressed to rvSO% of control by adenine (0.04 mg/ml) and by fraction 10 (0.04 mg/ml). These results suggest that B. abortus produces GMP and adenine and that these substances contribute to the intracellular survival of the bacteria.

Comments

This article is from Journal of Infectious Diseases 154(1986): 464.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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