Document Type

Article

Publication Version

Published Version

Publication Date

11-1985

Journal or Book Title

Journal of Infectious Diseases

Volume

152

Issue

5

First Page

913

Last Page

921

Abstract

The effects of fractions of Brucella abortus strain 2308 on functions of bovine polymorphonuclear neutrophils (PMNs) were examined in vitro. Ingestion of Staphylococcus aureus and reduction of nitroblue tetrazolium dye by bovine PMNs were not inhibited by heatkilled B. abortus. The ability of PMNs to iodinate proteins was significantly inhibited by live or heat-killed B. abortus and supernatant from heat-killed cells but not by washed heat-killed cells. Two inhibitory components isolated from the supernatant by highperformance liquid chromatography were characterized as nucleotide-like substances with molecular weights of <1,000. Inhibition of iodination by these components was concentration dependent. These results indicate that one of the mechanisms by which B. abortus may escape intracellular killing by PMNs is through the production of low-molecularweight components that inhibit the myeloperoxidase-hydrogen peroxide-halide antibacterial system of bovine PMNs.

Comments

This article is from Journal of Infectious Diseases 152 (1985): 913.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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