Document Type

Article

Publication Date

2006

Journal or Book Title

Developmental and Comparative Immunology

Volume

30

Issue

11

First Page

1060

Last Page

1069

DOI

10.1016/j.dci.2006.01.001

Abstract

Preterm infants experience enhanced susceptibility and severity to respiratory syncytial virus (RSV) infection. Terminal airway epithelium is an important site of RSV infection and the extent of local innate immune gene expression is poorly understood. In this study, expression of surfactant proteins A and D (SP-AD), sheep beta defensin 1 (SBD1), and toll-like receptor 4 (TLR4) mRNA were determined in whole lung homogenates from lambs. SP-AD and TLR4 mRNA expression increased (p<0.05) from late gestation to term birth. In addition, gene expression of LCM-retrieved type II pneumocytes (CD208+), adjacent epithelium (CD208−) and bronchial epithelium demonstrated that bronchiole-alveolar junction epithelium (combined CD208+/−) had significant (p<0.05) developmental increases in SP-AD, SBD1 and TLR4 mRNA, whereas CD208+ cells had statistically significant increases only with SP-A mRNA. Using immunofluorescence, SP-AD antigen distribution and intensity were also greater with developmental age. These studies show reduced SBD1, SP-AD, and TLR4 expression in the preterm lung and this may underlie enhanced RSV susceptibility.

Comments

This is a post-print of an article from Developmental and Comparative Immunology 30, no. 11 (2006): 1060–1069, doi:10.1016/j.dci.2006.01.001.

Copyright Owner

Elsevier Ltd.

Language

en

File Format

application/pdf

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