Document Type

Article

Publication Date

5-2004

Journal or Book Title

Clinical and Diagnostic Laboratory Immunology

Volume

11

Issue

3

First Page

599

Last Page

607

DOI

10.1128/CDLI.11.3.599–607.2004

Abstract

Defensins and surfactant protein A (SP-A) and SP-D are antimicrobial components of the pulmonary innate immune system. The purpose of this study was to determine the extent to which parainfluenza type 3 virus infection in neonatal lambs alters expression of sheep beta-defensin 1 (SBD-1), SP-A, and SP-D, all of which are constitutively transcribed by respiratory epithelia. Parainfluenza type 3 viral antigen was detected by immunohistochemistry (IHC) in the bronchioles of all infected lambs 3 days postinoculation and at diminished levels 6 days postinoculation, but it was absent 17 days postinoculation. At all times postinoculation, lung homogenates from parainfluenza type 3 virus-inoculated animals had increased SBD-1, SP-A, and SP-D mRNA levels as detected by fluorogenic real-time reverse transcriptase PCR. Protein levels of SP-A in lung homogenates detected by quantitative-competitive enzyme-linked immunosorbent assay and protein antigen of SP-A detected by IHC were not altered. These studies demonstrate that parainfluenza type 3 virus infection results in enhanced expression of constitutively transcribed innate immune factors expressed by respiratory epithelia and that this increased expression occurs concurrently with decreased viral replication.

Comments

This article is from Clinical and Diagnostic Laboratory Immunology 11, no. 3 (May 2004): 599–607, doi:10.1128/CDLI.11.3.599–607.2004.

Copyright Owner

American Society for Microbiology

Language

en

File Format

application/pdf

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