Campus Units

Zoology

Document Type

Article

Publication Version

Published Version

Publication Date

3-28-2003

Journal or Book Title

The Journal of Biological Chemistry

Volume

278

Issue

13

First Page

11696

Last Page

11704

DOI

10.1074/jbc.M213269200

Abstract

Using a yeast two-hybrid screen we have identified a novel isoform of the lola locus, Lola zf5, that interacts with the chromosomal kinase JIL-1. We characterized thelolalocus and provide evidence that it is a complex locus from which at least 17 different splice variants are likely to be generated. Fifteen of these each have a different zinc finger domain, whereas two are without. This potential for expression of multiple gene products suggests that they serve diverse functional roles in different developmental contexts. By Northern and Western blot analyses we demonstrate that the expression of Lola zf5 is developmentally regulated and that it is restricted to early embryogenesis. Immunocytochemical labeling with a Lola zf5-specific antibody of Drosophila embryos indicates that Lola zf5 is localized to nuclei. Furthermore, by creating double-mutant flies we show that a reduction of Lola protein levels resulting from mutations in the lola locus acts as a dominant modifier of a hypomorphic JIL-1allele leading to an increase in embryonic viability. Thus, genetic interaction assays provide direct evidence that gene products from the lola locus function within the same pathway as the chromosomal kinase JIL-1.

Comments

This article is published as Zhang, Weiguo, Yanming Wang, Jin Long, Jack Girton, Jørgen Johansen, and Kristen M. Johansen. "A Developmentally Regulated Splice Variant from the Complexlola Locus Encoding Multiple Different Zinc Finger Domain Proteins Interacts with the Chromosomal Kinase JIL-1." Journal of Biological Chemistry 278, no. 13 (2003): 11696-11704. doi: 10.1074/jbc.M213269200. Posted with permission.

Copyright Owner

The American Society for Biochemistry and Molecular Biology, Inc.

Language

en

File Format

application/pdf

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